Continued expansion with the addition of several world-class roller coasters was the theme of the 1990s for Busch Gardens Tampa.
- Attendance for the year estimated at 3.05, down slightly from the previous year due to lack of new attractions and overall slump in Florida tourism. (Orlando Sentinel 1/10/1991)
- May 23 – Questor opens to guests.
- August – Admission prices listed at $26.45 for ages 3 and above, with parking at $3. (Orlando Sentinel 8/9/1991)
- December – Admission prices raised about 4% to $28.70 including tax for adults, and $24.45 for children age 3 to 9. (Orlando Sentinel 7/17/1992)
- April 15 – Five gorillas arrive at the park from Yerkes Primate Center as part of the Myombe Reserve project.
- June 17 – Myombe Reserve officially opens to guests.
- July – Park hours were 9:30 to at least 6 p.m., with later hours for the summer. Daily admission is $26.95 plus tax, 22.95 plus tax for children age 3 to 9. Parking is $3. (The Morning Call, 7/19/1992)
- April 21 – Kumba officially opens.
- December 21 – Anheuser-Busch Brewery closes, part of corporate restructuring.
- May 16 – Montu officially opens to guests.
- October – Vice President of Marketing is quoted as saying park is on track to break attendance records for the year, estimated to be around 4 million. (Orlando Sentinel 10/8/1996)
- Attendance for the year estimated at 4.17 million, up 10% from previous year. (Orlando Sentinel 12/27/1996)
- July 4 – Edge of Africa officially opens to guests.
- July 15 – A press event is held to announce the addition of Gwazi, which would open the following June.
- October 19 – Park begins doing background checks (going back 7 years) on all new hires, regardless of role. Previously, only certain positions required background checks. (Orlando Sentinel 10/21/1998)
- Attendance for the year estimated at 4.2 million. (Ultimate Rollercoaster)
- June 18 – Gwazi officially opens to the public.